Heterorhabditis bacteriophora

Unit of packaging 
Heterorhabditis bacteriophora (entomopathogenic nematode) 
Standard pack size: bag in a box 
Contains: 50 million larvae (third stage) in inert carrier material 
Large pack size: 2 bags in a box 
Contains: 2 x 250 million larvae (third stage) in inert carrier material 

Larvae of various species of beetle, such as the black vine weevil Otiorhynchus sulcatus.

Preparation of spray solution

  • Put the contents of the pack into a bucket containing 5 litres of water (15-20°C)
  • Stir well and leave to soak for 20-30 seconds
  • Stir well once again and pour the entire solution into the spray tank
  • Top up until the required amount of solution is in the tank
  • Spray immediately after preparation
  • Use the whole pack in one go, as the distribution of nematodes in the pack is not homogenous!



interval (days)









curative (small-scale infestations)





large pack size: 1,000 m²/unit

curative (large-scale infestations)





large pack size: 500 m²/unit

Application and dose

  • With a watering- can, sprinkler system, backpack spray unit or vehicle-mounted spray unit
  • To avoid blockage, all filters should be removed
  • Use a maximum pressure of 200 P.S.I on spraying boom or pistol
  • The spray nozzle opening should be at least 0.5 mm (500 microns)
  • Spray over the soil surface
  • Continuous mixing should take place to prevent nematodes sinking to the bottom of the spray tank
  • Water the crop before and after application
  • Keep the soil moist for the first 2 weeks after application

Environmental conditions

  • The moisture content of the soil must be high
  • Soil temperature between 13 and 33°C

Storage and handling

  • Shelf life: see package for expiry date
  • Storage temperature: 2-6°C
  • In a dark place

Appearance Larvae (third stage): size 0.6 mm long
Other stages: develop inside host 

Mode of action 
After application, the nematodes actively search for their prey and penetrate them. The nematodes feed on the contents of the prey, excreting specific bacteria from their digestive tracts as they do so. These bacteria convert host tissue into products that the nematodes can easily ingest. The larvae die within a few days. 

Visible effects Affected larvae turn pink-red or even brown. 

The quantities of this product used should be adjusted to the mode of action of the product and the results that can be expected in the crop in which the product is applied. Your local Koppert consultant or recognised distributor will be able to advise you on the correct use. 
Only use products that are permitted in your country and the crop concerned. Check local registration requirements.

Koppert Biological Systems can not be held liable for unauthorised use.

Please contact your local consultant for further application information.

Side effects LARVANEM

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